Having issues with Index-hopping?
Having issues with Index-hopping? Unique Dual Indexes is a common approach used to mitigate Index-hopping that can be troublesome with some Illumina platforms. Unique dual indexing creates distinct, unrelated indexes in the p5 and p7 positions of the sequencing adaptors. If you only need 8 Unique Dual Index combinations, you can do that with Lucigen’s combinatorial unique dual indexing kit NxSeq Dual Indexing kit
You can generate up to 8 Unique Dual Indexed Libraries by using the following indexing primer pairs: (501, 701), (502, 702), (503, 703), (504, 704), (505, 705), (506, 706), (507, 707) and (508, 708). You would still have primers 709-712 that have not been used and the 501-508 primers. With the 709-712 primers you can create 2 more sets of primer pairs with 4 unique dual indexed combinations. Just remember you can’t combine libraries from group 1, 2 and 3. If you do you will not have Unique dual Indexes libraries in your sequencing pool.
NxSeq UltraLow DNA Library Kit, 96 Rxn
Unique Dual Indexes with Lucigen´s NxSeq UltraLow DNA Library Kit, 96 rxn
Interested in PCR-free Library kit needing only 75 ng sheared DNA as starting material?
NxSeq AmpFREE Low DNA Library Kit