EPICENTRE's revolutionary Nextera™ technology* uses in vitro transposition to prepare sequencer-ready libraries from genomic DNA for multiple sequencing platforms. The technology simultaneously fragments and tags DNA, in a single-tube reaction.
- Use nanogram amounts of starting DNA.
- Prepare sequencer-ready libraries in less than 2 hours.
- Incorporate platform-specific tags and optional barcodes.
- Validated on Roche 454™ GS FLX and GS FLX Titanium™, and Illumina Solexa® GAI, GAII
| Library Preparation Comparison |
| Processing Step |
Standard (~µg) |
Nextera™ (50 ng) |
| Fragmentation |
✓ (15-30 min) |
Add Transposomes
(5 min)
|
| Collection |
✓ (15 min) |
| Concentration |
✓ (15 min) |
| Size Selection |
✓ (60 min) |
| End-Repair |
✓ (60 min) |
| Clean-Up |
✓ (15 min) |
| A-Tailing |
+/- (30 min) |
| Adaptor Ligation |
✓ (60 min) |
| Clean-Up |
✓ (15 min) |
✓ (15 min) |
| Library Enrichment |
Variable (~60 min) |
PCR (~60 min) |
| Total Time: |
~6 Hours |
<2 Hours |
Contact: Oscar Ågerstam
Work flow:
