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T4 Polynucleotide Kinase Lucigen

Recombinant T4 Polynucleotide Kinase (T4 PNK).
•Applications include end-labeling and insert preparation for cloning.

T4 Polynucleotide Kinase catalyzes the transfer and exchange of gamma-phosphate of ATP to the 5´-hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates.

Source:
Purified from a strain of E. coli that carries a T4 Polynucleotide Kinase expression plasmid.

Applications:
• End-labeling DNA for probes and DNA sequencing (1)
• Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation (2)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:
• Ammonium ions are potent inhibitors of T4 Polynucleotide Kinase.  DNA should not be precipitated from or dissolved in buffers containing ammonium salts.
• Protruding 5’-overhangs are more efficiently phosphorylated than blunt- or recessed 5’ overhangs.

 

 

T4 Polynucleotide Kinase
Art. nr: 30061-3
Fp. Storlek 5,000 U
Lagerstatus Beställningsvara
4455 SEK
Antal:
T4 Polynucleotide Kinase
Art. nr: 30061-1
Fp. Storlek 1,000 U
Lagerstatus Beställningsvara
1029 SEK
Antal:
Förvaringsinfo

-20‡ C

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