RRBS (Reduced Representation Bisulfite Sequencing)

Single base pair resolution DNA methylation analysis.


Reduced Representation Bisulfite Sequencing (RRBS) is a method that facilitates the identification of differentially methylated regions by providing DNA methylation data at single base pair resolution on greater than 4 million CpGs. More cost effective than Whole Genome Bisulfite Sequencing and with greater coverage than bead array based DNA methylation platforms, RRBS is an ideal platform to profile DNA methylation across samples.


RRBS Highlights:

  • Data on 3 to 5 million CpGs per sample.
  • Biologically relevant target regions including promoters and CpG islands.
  • Sequencing depth of > 30,000,000 reads.
  • Low input DNA requirement of 1 µg.
  • Reduced library bias resulting from random bar code incorporation.

Deliverables:

  • Raw data (FASTQ) output files.
  • Aligned data (BAM) files.
  • Visualization files – BAM files can be used for visualization in IGV (Integrated Genome Viewer)
  • Methylation table includes percent methylation value for each CpG.
  • Differential methylation analysis
  • Figures & graphs – Includes coverage depth analysis, heatmaps & pie charts