A a serum-free medium for long-term freezing at -80ºC and preservation of valuable culture cells such as tumor cells and normal cells.

• Ready-to-use medium for preservation of cells
• Usable without diluting
• No program freezer is required

Operating Procedure: 1) Centrifuge the cells in logarithmic growth phase*, and collect 5 x 105 ~ 1 x 107 cells. 2) Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80ºC without preliminary freezing. The cells frozen at -80ºC can be preserved subsequently in liquid nitrogen. 3) Thawing must be carried out quickly in a constant-temperature chamber or a bath

*The cells must be frozen in logarithmic growth phase