It allows efficient cDNA synthesis and Real-Time PCR in a single tube. The kit includes a qPCR MasterMix (2x) to perform real-time PCR that contains all the reagent (except PCR primers, probe and template) needed for running PCR reactions. In addition, a separate RT Mix that comprises a balanced mixture of both RTase (Reverse Transcriptase) and RNase Inhibitor is also provided.
 
Additionally, the kit contain one sets of Primers and Fluorescent Probes to differentiate between 2019-nCoV and SARS-CoV (or bat SARS related CoV), and a Positive Control (PTC) and Negative Control (NTC).