Nextera™-Compatible Multiplex Primers (96 plex)
Active Motif
Art nr: 53155-AF
Active Motif
CUT&Tag-IT Assay Kits
A method to investigate genomic localization of histone modifications and some transcription factors that reveals interactions between proteins and DNA or identifies DNA binding sites for proteins of interest.
- Low background signal enables lower sequencing depth
- Sequencing-ready libraries without the laborious and costly steps of ChIP-Seq
Fresh (not frozen) unfixed cells are bound to concanavalin A beads and the antibody incubation is performed with cells in their native state, the chromatin is then digested and NGS libraries are prepared in a single step by tagmentation using the protein A-Tn5 (pA-Tn5) transposome enzyme that has been pre-loaded with sequencing adapters.
Download the CUT&Tag Complete Guide eBook for a comprehensive overview of the method
