The SalivaGene DNA stabilization buffer effects inactivation of DNases and prevents degradation of DNA, it preserves the microorganism titre and prelyses bacteria. The stabilized sample may be shipped in the tubes during 12 month at ambient temperature. The sample may be stored in the SalivaGene buffer at -20° for a several years. For the DNA extraction an aliquot may be used, the residual sample is stored for further extractions.
500 µl of the stabilized sample are used in the DNA isolation procedure. First the proteins are degraded with Proteinase K, followed by DNA-binding onto the membrane of the spin filter while contaminants pass though. After washing steps the DNA is eluted in either water or Elution Buffer D provided with the kit. The purified, high-quality DNA is ready to use for subsequent downstream applications:

• PCR applications
• genetic typing
• SNP and mutation analysis
• forensic analysis (e.g. screenings, paternity tests)
• pharmacogenomics