Promega

PureYield Plasmid Miniprep System

Up to 15μg of Transfection-Ready Plasmid from 3ml Cultures. Rapid, 10-minute miniprep protocol. Can be used with centrifugation or vacuum-based protocols, and includes a convenient lysis/neutralization indicator dye.

Can be used to isolate any plasmid from E. coli hosts but works most efficiently when the plasmid is up to 20,000bp in size. For best results, use plasmids that are 10,000bp or less.. When used for in vitro transcription reactions, the isolated plasmid DNA should be supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).