A-Plus Poly(A) Polymerase Tailing Kit

The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard protocol produces a poly(A)-tail length of ~150 b on 40-60 μg of RNA. Polyadenylation increases the stability of RNA in eukaryotic cells and enhances its ability to be translated after transfection or microinjection.