FUJIFILM Wako Chemicals Europe

Lysyl EndopeptidaseR (Lys-C)

Lysyl Endopeptidase, cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds.

Mw: 27,000 (gel filtration); 30,000 (SDS-PAGE)
Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 30 degrees C, pH 9.5
An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.