Promega
ProAlanase, MS Grade (0.2ug/ul)
ProAlanase is an endoprotease that preferentially cleaves proteins on the C-terminal side of proline and, to a lesser extent, alanine amino acids. Isolated and purified from the fungus Aspergillus niger, ProAlanase is also known as An-PEP or EndoPro. Peptides derived from protein digestion with ProAlanase are suitable for identification and characterization by mass spectrometry.
- Cleaves primarily C-terminal to proline and alanine residues
- Active at acidic pH (1–5.5) with a pH optimum of ~1.5
- Short digestion times of 1–2 hours
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