Masspektrometri

Suppress Artificial Deamidation and Disulfide Bond Scrambling in Protein Sample Prep

Serine Protease that Cleaves at C-Terminus of Tyr, Phe and Trp. Use alone or with other proteases for peptide mapping, peptide mass fingerprinting, MS/MS spectral matching

Characterize Protein Glycosylation. Enzymatic cleavage is between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, leaving one N-acetylglucosamine residue on the asparagine.

Protease for Challenging Protein Digestions. A highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins.

PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli.

Cleaves at the C-Terminus of Phe, Leu, Tyr, Trp. This protease can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Pepsin activity is optimal at pH 1.0–3.0

ProAlanase is an endoprotease that preferentially cleaves proteins on the C-terminal side of proline and, to a lesser extent, alanine amino acids. Isolated and purified from the fungus Aspergillus niger, ProAlanase is also known as An-PEP or EndoPro. Peptides derived from protein digestion with ProAlanase are suitable for identification and characterization by mass spectrometry.

For fast and efficient protein digestion with proteases such as Trypsin, Chymotrypsin and Lys-C. For in-gel protein digestion, ProteaseMAX™ Surfactant offers time and labor savings. Digestion step is complete in 1 hour, and the surfactant provides concurrent extraction of peptides from gels, eliminating the need for post-digestion peptide extraction.

Rapid-Digestion Trypsin and Rapid-Digestion Trypsin/Lys-C Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4–18 hours required for trypsin digestion. Both kits contain three components: i) protease (Trypsin or Trypsin/Lys-C Mix); ii) protease Resuspension Buffer; and iii) Rapid-Digestion Buffer optimized for faster protein digestions. simple-to-use protocol that is both fast and efficient

Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion

Trypsin for Most Accurate Biotherapeutic Protein Characterization and Specialized Proteomics Applications

Improve Protein Digestion with Mix of Trypsin Gold and rLys-C where the Trypsin Gold is recombinant version free of non-specific cleavage activity.

Hydrolyzes Peptide Bonds N-Terminal to Aspartic Acid Residues. A highly active protease suitable for proteomic analysis of complex mixtures as well as peptide mapping of purified proteins, such as therapeutic monoclonal antibodies.