Enzymer

Exonuclease III is a 3' --> 5' exonuclease specific for dsDNA.

TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'-->3' direction. Recombinant.

A DNA-dependent DNA polymerase that lacks the 5'-->3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'-->3' polymerase, 3'-->5' exonuclease and strand displacement activities.

Point mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.

Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.

RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.

Helpful for Preparing Long cDNAs and Libraries with a High Percentage of Full-Length cDNA

The 10X Flexi® Enzyme Blend (SgfI and PmeI) is required to screen Flexi® Vector clones for the presence of the protein-coding region of interest. This enzyme blend can also be used to cleave PCR products prior to cloning into the Flexi® Vectors or transfer protein-coding regions between Flexi® Vectors. 

Aliquots of 10X Restriction Enzyme Buffers A, B, C and D

The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard protocol produces a poly(A)-tail length of ~150 b on 40-60 μg of RNA. Polyadenylation increases the stability of RNA in eukaryotic cells and enhances its ability to be translated after transfection or microinjection.

Add poly(A) tails to the 3' ends of RNA molecules, including in vitro transcribed RNAs, with this robust kit.

Efficient Reverse Transcription of Templates with High Secondary Structure

Ampligase Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures.

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed.

Capable of digesting DNA in 15 minutes or less. Active and capable of digestion directly in GoTaq® Green Master Mix. Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications.

Genome qualified to ensure optimal performance in genomic analysis applications

Genome qualified to ensure optimal performance in genomic analysis applications

Easily Screen and Profile CDK2/CyclinA2 Kinase Inhibitors Includes kinase, substrate and reaction buffer

For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.

Thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA