Proteininteraktioner

Powerful System for Detecting Protein:Protein Interactions In Vivo

Enables a range of functionalities, including affinity labeling for protein purification. These ligands form highly specific and nearly irreversible covalent bonds with HaloTag® fusion proteins under physiological conditions, ensuring stable and efficient labeling with minimal background interference.

The NanoBRET Nano-Glo Detection System provides the NanoBRET Nano-Glo Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag NanoBRET 618 Ligand for the fluorescent energy acceptor.

The NanoBRET™ Nano-Glo(R) Substrate is the substrate used by NanoLuc(R) Luciferase to generate the donor signal in several NanoBRET™ Assays.

For detection of Protein:Protein Interactions in Live Cells

NanoBRET™ assay technology is dependent upon energy transfer from a luminescent donor (NanoLuc® luciferase) to a fluorescent acceptor (HaloTag® NanoBRET™ 618 Ligand). NanoLuc® luciferase HaloTag® protein are fused to the target proteins of interest and fusion proteins expressed at low cellular levels, enabling monitoring and screening studies of protein interactions that reflect true cellular physiology. The NanoBRET™ Assay is fully reversible, enabling studies of both induction and inhibition of protein interactions. 

Sensitive Detection of Protein Interactions in Live Cells