Life Science

Nano-Glo HiBiT Extracellular Detection System quantitates cell surface or secreted protein expression using a simple add-mix-read assay format and the nonlytic Nano-Glo HiBiT Extracellular Detection Reagent.

The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay forma

The Nano-Glo Live Cell Assay System is a single-addition, non-lytic detection reagent used to measure NanoBiT or NanoLuc luminescence from living cells.

The Nano-Glo Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.

The NanoBRET Nano-Glo Detection System provides the NanoBRET Nano-Glo Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag NanoBRET 618 Ligand for the fluorescent energy acceptor.

The NanoBRET™ Nano-Glo(R) Substrate is the substrate used by NanoLuc(R) Luciferase to generate the donor signal in several NanoBRET™ Assays.

For detection of Protein:Protein Interactions in Live Cells

NanoBRET Target Engagement Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. The assay is based on NanoBRET, an energy transfer technique for measuring molecular proximity in living cells.

NanoBRET Target Engagement Intracellular HDAC Assay measures compound binding at select HDAC target proteins within intact cells. The assay is based on NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.

The NanoBRET Target Engagement (TE) Assay measures compound binding at select kinase targets within intact cells. The assay is based on the NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.

The NanoBRET Target Engagement (TE) Assay measures compound binding at select kinase targets within intact cells. The assay is based on the NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells.

NanoBRET™ assay technology is dependent upon energy transfer from a luminescent donor (NanoLuc® luciferase) to a fluorescent acceptor (HaloTag® NanoBRET™ 618 Ligand). NanoLuc® luciferase HaloTag® protein are fused to the target proteins of interest and fusion proteins expressed at low cellular levels, enabling monitoring and screening studies of protein interactions that reflect true cellular physiology. The NanoBRET™ Assay is fully reversible, enabling studies of both induction and inhibition of protein interactions. 

Sensitive Detection of Protein Interactions in Live Cells