Epigenetik

A synthetic peptide derived from Histone H3.1, amino acids 1-21, modified by methylation on lysine 9, with an added lysine residue containing a biotin moiety and a C-terminal amide group (CONH2). C-term biotinylated Lys residue is separated with two Gly (provide short spacer) and one Tyr (provides accurate UV quantification) from the rest of the peptide sequence. The presence of the lysine residue is for quantitation and does not affect peptide functionality.

A synthetic peptide derived from Histone H3.1, amino acids 1-21, modified with an acetyl group on lysine 9, with an added lysine residue containing a biotin moiety and a C-terminal amide group (CONH2). C-term biotinylated Lys residue is separated with two Gly (provide short spacer) and one Tyr (provides accurate UV quantification) from the rest of the peptide sequence. The presence of the lysine residue is for quantitation and does not affect peptide functionality.

Applications Validated by Active Motif: ChIP: 2 - 10 µl per ChIP ChIP-Seq: 10 µl each ICC/IF: 1:500 - 1:1,000 dilution WB*: 1:1,000 - 1:5,000 dilution CUT&Tag: 1 µl per 50 µl reaction

This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3. Polyclonal antibody raised in rabbit.