Epigenetik

Human Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with human samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple human cell lines.

Efficient enrichment of 5-mC containing DNA fragments

Active Motif’s MethylCollector™ MBD Capture Kit provides an efficient method for isolating CpG-methylated DNA from limited amounts of cell or tissue samples

Methylated Human Control DNA is purified from a human male source. CpG sites in the DNA are enzymatically methylated by M. SssI methyltransferase to provide a high percentage of methylated CpG motifs. Methylated Human Control DNA can be bisulfite-converted with MethylEdge™ Bisulfite Conversion System in parallel with experimental samples to assess conversion efficiency.