HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

HB101 Competent Cells are prepared according to a modified procedure of Hanahan.