Biosearch Technologies (Lucigen)
MasterPure Yeast DNA Purification Kit
Efficient, high-yield purification of high-molecular-weight DNA from yeast and other fungi. The protocol involves nonenzymatic cell lysis at 65°C, followed by removal of protein by precipitation, and nucleic acid precipitation and resuspension.
- Obtain purified DNA in less than 40 minutes
- The average total yield of DNA from 1.5 mL of liquid culture is 3-4 μg for Saccharomyces species and 6-8 μg for Candida species
- Purify DNA from a wide variety of yeast species, including Candida, Saccharomyces, Pichia, and Schizosaccharomyces, and filamentous fungi such as Aspergillus and Penicillium